Spontaneous Reperfusion after In Situ Thromboembolic Stroke in Mice |
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Authors: | Anne Durand Fabien Chauveau Tae-Hee Cho Radu Bolbos Jean-Baptiste Langlois Laure Hermitte Marlène Wiart Yves Berthezène Norbert Nighoghossian |
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Institution: | 1. Université de Lyon, CREATIS, CNRS UMR5220, INSERM U1044, INSA-Lyon, Université Lyon 1, Hospices Civils de Lyon, Lyon, France.; 2. CERMEP-Imagerie du Vivant, Animage, Lyon, France.; Charité Universitaetsmedizin Berlin, Germany, |
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Abstract: | Injection of thrombin into the middle cerebral artery (MCA) of mice has been proposed as a new model of thromboembolic stroke. The present study used sequential multiparametric Magnetic Resonance Imaging (MRI), including Magnetic Resonance Angiography (MRA), Diffusion-Weighted Imaging (DWI) and Perfusion-Weighted Imaging (PWI), to document MCA occlusion, PWI-DWI mismatch, and lesion development. In the first experiment, complete MCA occlusion and reproducible hypoperfusion were obtained in 85% of animals during the first hour after stroke onset. In the second experiment, 80% of animals showed partial to complete reperfusion during a three-hour follow-up. Spontaneous reperfusion thus contributed to the variability in ischemic volume in this model. The study confirmed the value of the model for evaluating new thrombolytic treatments, but calls for extended MRI follow-up at the acute stage in therapeutic studies. |
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