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Development of human monoclonal antibodies: A review
Authors:Toni Lindl
Institution:(1) Institute of Applied Cell Culture, D-81669 München, Germany
Abstract:This article describes the current status in the development of human monoclonal antibodies. Over the last ten years a lot of information about the human immune system has emerged. Combining these with the many new (bio-)technologies it is plausible that the long awaited breakthrough of this technology is close. This paper focuses on the ldquoclassicalrdquo cell-biological methods of achieving stable, antibody-producing human cell lines via cell fusion methods or virus derived transformations of human B-lymphocytes, as well as genetic engineering methods e.g. DNA libraries or phage display technology. The available in vitro immunization methods are critically reviewed and their impact on this topic is discussed. Therapeutic applications for cancer treatment or passive immunization against infectious diseases with antibodies derived by both ways are also reviewed.
Keywords:human monoclonal antibodies  in vitro immunization  cell fusion  heterohybridoma  Epstein-Barr virus transformation  human immunoglobulin libraries  phage display technology
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