A fluorimetric method for continuously assaying ATPase: application to small specimens of glycerol-extracted muscle fibers. |
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| Authors: | R Takashi S Putnam |
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| Affiliation: | Cardiovascular Research Institute, University of California, San Francisco, California 94143 USA |
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| Abstract: | A continuous fluorimetric method using auxiliary-coupling enzymes such as pyruvate kinase and lactate dehydrogenase for measuring ADP production to assay ATPase activity is described. This method is simpler, more rapid, and more sensitive than the previously used spectrophotometric method. The application of this method for studying the ATPase of rabbit psoas muscle fibers during Mg2+-ATP activation is also illustrated and discussed. |
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