Continuous production of l-serine by immobilized growing Corynebacterium glycinophilum cells |
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| Authors: | Takaaki Tanaka Keiichi Yamamoto Sirintornthep Towprayoon Hiroki Nakajima Kenji Sonomoto Kenzo Yokozeki Koji Kubota Atsuo Tanaka |
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| Institution: | (1) Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Yoshida Sakyo-ku, 606 Kyoto, Japan;(2) Central Research Laboratories of Ajinomoto Co., Inc., 1-1, Suzuki-cho, 210 Kawasaki, Kanagawa, Japan;(3) Present address: Department of Biology, Faculty of Science, Kohn Kaen University, 40002 Khon Kaen, Thailand |
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| Abstract: | Summary Auxotrophic mutant cells of Corynebacterium glycinophilum with high l-serine production activity were immobilized by entrapment with various gel materials, such as synthetic prepolymers and natural polysaccharides. The entrapped cells were used for estimation of l-serine productivity in a medium supplemented with glycine as a precursor. Based on the above criteria, including cell growth in gels and cell leakage from gels, calcium alginate was the most suitable gel material. Continuous l-serine fermentation with calcium alginate-entrapped growing cells was successfully achieved in an air-bubbled reactor for at least 13 days. |
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