Genes of the constant regions of functional immunoglobulin heavy chain of Japanese flounder,<Emphasis Type="Italic"> Paralichthys olivaceus</Emphasis> |
| |
Authors: | Prapansak?Srisapoome Tsuyoshi?Ohira Ikuo?Hirono Email author" target="_blank">Takashi?AokiEmail author |
| |
Institution: | (1) Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, 108-8477 Tokyo, Japan |
| |
Abstract: | IgM and IgD genes of the Japanese flounder were cloned and characterized from a genomic bacterial artificial chromosome (BAC) library. The gene contained four constant region exons (C 1–C 4), and two transmembrane exons ( TM1 and TM2), which conforms to the organizational pattern of all other vertebrate -chain genes examined so far. In the same BAC clone, the gene, which is homologous to the IgD gene in mammals and teleost fish, was found immediately (0.9 kb) downstream of the IgM gene. This gene encoded seven exons (C 1–C 7) and two TM exons ( TM1 and TM2) and had no duplication of C 1-C 2, as found in Atlantic cod, or C 2-C 3-C 4, as found in Atlantic salmon and channel catfish. Phylogenetic and sequence analyses strongly suggest that teleost is more closely related to non-IgM isotypes than IgM isotypes. The heavy chain (IgH) locus of Japanese flounder, which encodes m, s and m, was found to be fully functional. |
| |
Keywords: | Japanese flounder IgM IgD Constant region Gene organization |
本文献已被 PubMed SpringerLink 等数据库收录! |
|