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Isolation of the plastid FtsZ gene from Cyanophora paradoxa (Glaucocystophyceae, Glaucocystophyta)
Authors:Mayuko Sato  Toshikazu Nishikawa  Tomokazu Yamazaki  Shigeyuki Kawano
Affiliation:Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Building FSB-601, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
Abstract:Plastids of glaucocystophytes are termed cyanelles and retain primitive features, such as a peptidoglycan wall. We isolated a full‐length prokaryotic plastid division gene, FtsZ, from the glaucocystophyte alga Cyanophora paradoxa Korshikov (CpFtsZ‐cy). CpftsZ‐cy has a chloroplast‐targeting signal at the N‐teminus. Immunofluorescence microscopy showed that CpFtsZ‐cy forms a ring‐like structure at the division plane of cyanelles.
Keywords:cyanelle    Cyanophora paradoxa    FtsZ    Glaucocystophyta    plastid division
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