几种经济作物根际拮抗细菌的多样性

采用抗菌谱测定以及BOXAIR-PCR、生理生化特征和16S rDNA序列分析等方法对分离自10种作物根际的55株拮抗细菌的多样性及主要拮抗菌类群进行了分析.结果表明： 根际拮抗细菌的拮抗作用具有丰富的多样性；BOXAIR-PCR分析中供试菌在721％相似性水平上可以聚为7个群，850%相似性水平上聚为25个群；所有供试根际拮抗菌分别属于芽孢杆菌属（Bacillus）芽孢杆菌属（Paenibacillus）、短芽孢杆菌属（Brevibacillus）、假单胞菌属（Pseudomonas）和产碱菌属（Alcaligenes）.作物根际拮抗菌具有丰富的遗传多样性和拮抗性能多样性.

Diversity of antagonistic bacteria isolated from rhizosphere of several cash crops

By adopting antimicrobial spectrum test, BOXAIR-PCR, physiological and biochemical, and 16S rDNA sequencing analysis, this paper analyzed the diversity of 55 antagonistic bacterial strains isolated from the rhizosphere of 10 cash crops. There was a high diversity of the antagonism of the strains. Based on BOXAIR-PCR, all the strains were clustered into 7 groups at the similarity level of 72.1%, and divided into 25 groups at the similarity level of 85.0%. All the strains belonged to Bacillus, Paenibacillus, Brevibacillus, Pseudomonas and Alcaligenes, respectively. The antagonistic bacteria isolated from the rhizosphere had high genetic diversity and high diversity in antagonistic activity.