In vitro differentiation in callus cultures of moth bean,Vigna aconitifolia (JACQ) marechal |
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Authors: | S. Bhargava N. Chandra |
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Affiliation: | 1. Department of Botany, University of Rajasthan, 302004, Jaipur, India
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Abstract: | Callus cultures of two cultivars of Vigna aconitifolia (IPCMO-926, RDM-120) were raised and their growth and differentiation studied. In IPCMO-926 callus cultures, numerous shoot buds differentiated on MS medium with BA (0.4–22.2 μM) alone or in combination with IAA (5.7 μM). In RDM-120 best differentiation of shoot buds was observed on a medium with K (23.2 μM) and IAA (5.7 μM). Kinetin alone, however, induced rhizogenesis in callus cultures. In suspension cultures of IPCMO-926 embryoids differentiated on MS medium with K (0.5 μM) and 2,4-D (0.4 and 0.9 μM). |
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