Haploid plants from in vitro anther culture of Annona squamosa Linn |
| |
Authors: | S. Nair P. K. Gupta A. F. Mascarenhas |
| |
Affiliation: | 1. Biochemistry Division, National Chemical Laboratory, Pune 411 008, India
|
| |
Abstract: | Haploid plants were induced from anther callus of Annona squamosa Linn. (Custard apple) on a Nitsch basal medium supplemented with 6-benzyl-aminopurine and naphthalene acetic acid. When naphthalene acetic acid was replaced by indole-3-acetic acid only multiple shoots were obtained. Pretreatment (chilling, centrifugation, reduced atmospheric pressure etc.) of the flowers was not effective but dissection of the flowers in a suspension of activated charcoal and sucrose was found essential. The anthers required an initial dark period and a high sucrose medium followed by light and lowered sucrose levels. Root tip squashes of the regenerated plantlets revealed the haploid (n = 7) nature of the plantlets. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |