Role of cortical microtubules in the orientation of cellulose microfibril deposition in higher-plant cells |
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Authors: | S Hasezawa and H Nozaki |
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Institution: | (1) Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Hongo, Bunkyo-ku, 113-0033 Tokyo, Japan;(2) Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Hongo, Tokyo |
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Abstract: | Summary Cortical microtubules (MTs) have been implicated in the morphogenesis of plant cells by regulating the orientation of newly
deposited cellulose microfibrils (CMFs). However, the role of MTs in oriented CMF deposition is still unclear. We have investigated
the mechanism of CMF deposition with cultured tobacco protoplasts derived from taxol-treated BY-2 cells (taxol protoplasts).
The BY-2 protoplasts regenerated patches of β-l,3-glucan (callose) and fibrils of β-l,4-glucan (cellulose). Taxol protoplasts
possessed the same ordered MT arrays as material cells and regenerated CMFs with patterns almost coincidental with MTs. Electron
microscopy revealed that, on the surface of cultured taxol protoplasts, each CMF bundle appeared to be deposited on each cortical
MT. These results suggest that MTs may attach directly to the cellulose-synthesizing complexes, by some form of linkage, and
regulate the movement of these complexes in higher-plant cells. |
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Keywords: | Cellulose microfibril Cellulose-synthesizing complex Cortical microtubule Protoplast Taxol Tobacco BY-2 cells |
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