A physical map of belomycin-specific fragmentation sites on PM2 bacteriophage DNA |
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| Authors: | R Stephen Lloyd Charles W Haidle Donald L Robberson Marion L Dodson Jr |
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| Institution: | (1) Department of Biology, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, 77030 Houston, Texas, USA;(2) Environmental Science Park, Research Division, The University of Texas System Cancer Center, 78957 Smithville, Texas, USA |
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| Abstract: | Bleomycin treatment of PM2 DNA results in fragmentation of the genome at several specific sites. Application of restriction
endonuclease digestion followed by bleomycin treatment has provided the basis for constructing a physical map of bleomycin
fragmentation sites. Eleven sites have been located on the physical map relative toHpa II,Pst I, andHindIII cleavage sites. The fragmentation sites are not clustered in a particular region of the PM2 genome but 3 of the 11 sites
do occur between theHpa II andPst I cleavage sites, a segment of DNA which comprises 14% of the PM2 DNA length. |
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