| 一种内源性的竞争性的γ-氨基丁酸受体抑制剂 |
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| 引用本文： | 蔡宁生,张兆耕,王芳,周廷冲. 一种内源性的竞争性的γ-氨基丁酸受体抑制剂[J]. 中国生物化学与分子生物学报, 1987, 3(4): 337-343 |
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| 作者姓名： | 蔡宁生 张兆耕 王芳 周廷冲 |
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| 作者单位： | 军事医学科学院基础医学研究所 北京(蔡宁生，张兆耕，王芳)，军事医学科学院基础医学研究所 北京(周廷冲) |
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| 基金项目： | 中国科学院科学基金生准字152号 |
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| 摘 要： | 用离心、超滤、分子筛及高压液相色谱技术从牛小脑得到一种GABA受体结合抑制剂。该物质竞争性地抑制~3H-蝇草醇(Muscimol)与突触膜GABA受体的高亲和力部位结合,Ki值为0.73±0.25ng。对~3H-二苯羟乙酸-3-奎宁环脂(QNB)和~3H-氟硝安定(~3H-FNZP)与脑匀浆和突触膜的结合没有影响。初步分析该物质为分子量6000道尔顿的肽。
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| 关 键 词： | GABA受体 内源性抑制剂 牛小脑 ~3H-Muscimol竞争性抑制 |
| 收稿时间： | 1987-08-20 |
AN ENDOGENOUS, COMPETITIVE GABA-RECEPTOR INHIBITOR |
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| Cai,Ning-Sheng Zhang,Zhao-Geng Wang,FangZhou,Ting-Chong. AN ENDOGENOUS, COMPETITIVE GABA-RECEPTOR INHIBITOR[J]. Chinese Journal of Biochemistry and Molecular Biology, 1987, 3(4): 337-343 |
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| Authors: | Cai Ning-Sheng Zhang Zhao-Geng Wang FangZhou Ting-Chong |
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| Affiliation: | (Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing |
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| Abstract: | An endogenous inhibitor of GABA-receptor has been isolated from bovine cerebellum extract and purified by centrifugation, superfiltation, SephadexG-100 Chro-matography and HPLC Separation.This endogenous inhibitor competitively inhibited the high-affinity binding of 3H-Muscimol to crude synaptic membrane from bovine cerebellum with K1 = 0.73 ±0.25ng but failed to influence the binding of 3H-QNB and 3H-FNZP to brain homogenates and synaptic membrane. Preliminary examination showed that this inhibitor may by a peptide of MW 6,000 dal. |
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| Keywords: | GABA-Receptor endogenous GABA-R inhibitor competitive inhibition bovine cerebellum 3H-Muscimol |
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