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Postsynthetic Modification of Oligonucleotides with Imidazophenazine Dye and its Effect on Duplex Stability |
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| Authors: | Mrs Larysa Dubey Dr Olga Ryazanova Dr Victor Zozulya Dr Dmytro Fedoryak Dr Igor Dubey |
| Institution: | 1. Institute of Molecular Biology and Genetics, National Academy of Sciences , Kyiv , Ukraine;2. Institute for Low Temperature Physics and Engineering, National Academy of Sciences , Kharkov , Ukraine;3. Institute of Bioorganic and Petroleum Chemistry, National Academy of Sciences , Kyiv , Ukraine |
| Abstract: | Carboxyalkyl derivative of the intercalating agent imidazo4,5-b]phenazine was used for the postsynthetic oligonucleotide modification. Model pentadecathymidylate-imidazophenazine conjugate was prepared from 5′-aminoalkyl-modified (dT)15 by using phosphonium coupling reagent BOP in the presence of 1-hydroxybenzotriazole. Spectral-fluorescent properties of the conjugate were studied. The attachment of the dye was found to increase the thermal stability of (dT)15 duplex with poly(dA) by more than 4°C, probably by the intercalation mechanism. |
| Keywords: | Oligonucleotide conjugates intercalating agents postsynthetic modification imidazophenazine DNA duplex stability |