Stereoselective Nucleoside Deuteration for NMR Studies of DNA |
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Authors: | Mark Lukin Carlos de los Santos |
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Institution: | Department of Pharmacological Sciences , Stony Brook University , Stony Brook, New York, USA |
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Abstract: | A procedure has been elaborated for stereoselective deuterium substitution of one of the diastereotopic 5′-protons in 2′-deoxynucleotides. The synthetic scheme uses the reduction of the 5-oxosugar derivative with deuterated Alpine-Borane. The resulting deuterosugar is converted into pyrimidine nucleosides and incorporated into DNA using standard protocols. Comparison of two-dimensional NMR spectra of the fully protonated and partially deuterated duplexes allowed us to assign diastereotopic 5′ protons, increasing the number of experimental restraints used for structure determination. |
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Keywords: | Deuterated nucleosides oligonucleotides solution state NMR DNA lesions |
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