Enhancement of Electrochemical Differentiation Ability of Nucleobases in Phosphate Buffer Solution at pH 7.4 |
| |
| Authors: | Jingjie Cui Dehui Sun Xiaoshan He Guancong Wang Hongshi Zhao Hong Liu |
| |
| Institution: | 1. State Key Laboratory of Crystal Materials, Center of Bio &2. Micro/Nano Functional Materials , Shandong University , Jinan , PR China;3. College of Life Information Science &4. Instrument Engineering , Hangzhou Dianzi University , Hangzhou , China;5. Micro/Nano Functional Materials , Shandong University , Jinan , PR China;6. Department of Applied Physics , Chongqing University , Chongqing , PR China |
| |
| Abstract: | The electrochemical behavior of nucleobases has been studied in 0.1 M phosphate buffer solution at pH 7.4, using a bare graphite electrode. Guanine and adenine produced well-defined oxidation peaks at about +0.63 and +0.91 V at 100 mV/s, respectively. Nucleobases exhibit an irreversible and hybrid-controlled electrochemical process, including adsorption and diffusion. The nucleobase oxidation peaks shift due to the selective interactions of nucleobases with each other. The oxidation peaks for three different pyrimidine bases, uracil, cytosine, and thymine, can be clearly identified at +1.26, +1.41, and +1.32 V, respectively. These differences in the electrochemical behavior among nucleobases can be attributed to their different chemical structures. |
| |
| Keywords: | Nucleobases electrochemical detection phosphate buffer solution of pH 7 4 selective interactions graphite electrode |
|
|
