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Probing the MvaI Methyltransferase Region that Interacts with DNA: Affinity Labeling with the Dialdehyde-Containing DNA Duplexes |
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| Authors: | O. M. Gritsenko S. N. Mikhailov E. V. Efimtseva A. Van Aerschot P. Herdewijn E. S. Gromova |
| Affiliation: | 1. Department of Chemistry , Moscow State University , Moscow , 119899 , Russia;2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences , Vavilov str. 32, Moscow , 117984 , Russia;3. Rega Institute, Katholieke Universiteit Leuven , Minderbroedersstraat 10, B-3000 , Leuven , Belgium |
| Abstract: | Abstract Affinity labeling of methyltransferase MvaI by DNA duplexes containing oxidized 2′-O-β-D-ribofuranosylcytidine or 1-(β-D-galactopyranosyl)thymine residues was performed. Partial chemical hydrolysis of the covalently bound methylase in the conjugates with the dialdehyde-containing DNA allowed us to determine the amino acid region in the C terminus of methylase MvaI that interacts with DNA. |
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