Invasion of Strongly Binding Oligonucleotides into tRNA Structure |
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| Authors: | V Petyuk R Serikov V Tolstikov V Potapov R Giege M Zenkova |
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| Institution: | 1. Laboratory of Nucleic Acids Biochemistry, Novosibirsk Institute of Bioorganic Chemistry , 8 Lavrentiev avenue, 630090, Novosibirsk, Russia;2. Novosibirsk State University , 2, University av., 630090, Russia;3. Laboratory of Structure and Function of Human Genes, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry , UI. Miklukho-Maklaya 16/10, Moscow, 117871, Russia;4. Département “Mécanismes et Macromolécules de la Synthèse Protéquie et Cristallogènese” , UPR 9002 ‘SMBMR’, Institut de Biologie Moléculaire et Cellulaire du CNRS , 15 rue René Descartes, 67084, Strasbourg Cédex, France |
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| Abstract: | Abstract Interaction of yeast tRNAPhe with oligodeoxyribonucleotides containing 5-methylcytosine, 2-aminoadenine, and 5-propynyl-2′-deoxyuridine was investigated. The modified oligonucleotides show increased binding capacity although the association rates are similar for the modified and natural oligonucleotides. The most pronounced increase in association constant (70 times) due to the incorporation of the strongly binding units was achieved in the case of oligonucleotide complementary to the sequence 65–76 of the tRNAphe. |
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