Solution Structure of a GAAG Tetraloop in Helix 6 of SRP RNA from Pyrococcus furiosus |
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| Authors: | Kiyoshi Okada Mari Takahashi Taiichi Sakamoto Gota Kawai Kouji Nakamura Akio Kanai |
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| Institution: | 1. Department of Life and Environmental Sciences, Faculty of Engineering , Chiba Institute of Technology , Chiba , Japan;2. Department of Life and Environmental Sciences, Faculty of Engineering , Chiba Institute of Technology , Chiba , Japan;3. Protein Research Group , RIKEN Genomic Sciences Center , Kanagawa , Japan;4. Institute of Biological Sciences, University of Tsukuba , Ibaraki , Japan;5. Institute for Advanced Biosciences, Keio University , Yamagata , Japan |
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| Abstract: | The NMR structure of a 12-mer RNA derived from the helix 6 of SRP RNA from Pyrococcus furiosus, whose loop-closing base pair is U:G, was determined, and the structural and thermodynamic properties of the RNA were compared with those of a mutant RNA with the C:G closing base pair. Although the structures of the two RNAs are similar to each other and adopt the GNRR motif, the conformational stabilities are significantly different to each other. It was suggested that weaker stacking interaction of the GAAG loop with the U:G closing base pair in 12-mer RNA causes the lower conformational stability. |
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| Keywords: | SRP GNRR motif |
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