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Reagents For The Selective Immobilization Of Oligonucleotides On Solid Supports |
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| Authors: | Maksim V Kvach Igor A Prokhorenko Alexey V Ustinov Sergei V Gontarev Vladimir A Korshun Vadim V Shmanai |
| Institution: | 1. Institute of Physical Organic Chemistry NAS , Minsk, Belarus;2. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry , Moskow, Russia |
| Abstract: | New reagents (CPGs and phosphoramidites) for automatic solid phase synthesis of modified oligonucleotides were designed. Three oligonucleotides carrying fluorescent label at the 5′-terminus and an anchor group at the 3′-terminus were prepared and their immobilization in orthogonal conditions on solid supports was studied. |
| Keywords: | Oligonucleotides immobilization click chemistry streptavidin-biotin |