Modulation of AMP Deaminase in Rat Hearts Subjected to Ischemia and Reperfusion by Purine Riboside |
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| Authors: | T. Borkowski M. Lipinski R. Kaminski E. Krzyminska-Stasiuk M. Langowska G. Raczak |
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| Affiliation: | 1. Department of Biochemistry , Medical University of Gdansk , Gdansk, Poland;2. Department of Pharmaceutical Biochemistry , Medical University of Gdansk , Gdansk, Poland;3. Clinic of Cardiology and Electrotherapy , Medical University of Gdansk , Gdansk, Poland |
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| Abstract: | Changes in AMP deaminase (AMPD) activity influence heart function and progression of heart disease, but the underlying mechanism is unknown. We evaluated the effect of purine riboside (Purr) on the activity of AMPD in perfused rat hearts and in isolated rat cardiomyocytes. Brief perfusion of the pre-ischemic heart with 200 μ M Purr resulted in activation of AMPD, more pronounced degradation of the adenine nucleotides, and reduced recovery of the adenine nucleotide pool during reperfusion. Brief incubation of rat cardiomyocytes with 200 μ M Purr also activated AMPD, while prolonged exposure resulted in enzyme inhibition. We conclude that Purr activates AMPD, whereas metabolites of this compound may inhibit the enzyme. |
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| Keywords: | AMP deaminase heart cardiomyocytes rat purine riboside ischemia-reperfusion |
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