Apoptotic Effect of Ganciclovir on Primary Effusion Lymphoma Cells Infected with Kaposi's Sarcoma-Associated Herpesvirus |
| |
Authors: | Masahiro Fujimuro Hisanori Inoue Yasuhiro Teishikata Yuriko Ishida Hideyoshi Yokosawa |
| |
Institution: | Department of Biochemistry , Graduate School of Pharmaceutical Sciences, Hokkaido University , Sapporo , Japan |
| |
Abstract: | We evaluated the cytotoxic and apoptotic effects of two purine nucleoside analogues, acyclovir (ACV) and ganciclovir (GCV), on lymphoma cells stably harboring Kaposi's sarcoma-associated herpesvirus (KSHV). Colorimetric caspase assay, flow cytometry, and immunoblotting with antibodies against apoptosis-related molecules revealed that GCV has cytotoxic activity toward KSHV-infected primary effusion lymphoma cells, while ACV has weak or little activity. In addition to the GCV-induced cytotoxicity, apoptosis via caspase-7/8, cleavage of poly(ADP-ribose) polymerase, and accumulation of p53 and p21 were induced by GCV treatment. In contrast, neither ACV nor GCV have cytotoxicity- or apoptosis-inducing activities toward uninfected cells. |
| |
Keywords: | Epstein-barr virus (EBV) Kaposi's sarcoma-associated herpesvirus (KSHV) Human herpesvirus 8 (HHV-8) Acyclovir (ACV) Ganciclovir (GCV) |
|
|