Novel Neo Glycolipid: Formulation into Pegylated Cationic Liposomes and Targeting of DNA Lipoplexes to the Hepatocyte-Derived Cell Line HepG2 |
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| Authors: | Nicolisha Narainpersad Moganavelli Singh Mario Ariatti |
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| Affiliation: | Department of Biochemistry, Non-Viral Gene Delivery Laboratory , University of KwaZulu-Natal, Westville Campus , Durban , South Africa |
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| Abstract: | Liver parenchymal cells are an important target for the treatment of several metabolic and viral disorders. Corrective gene delivery for this purpose is an avenue that is receiving increasing attention. In the present study, we report a novel neo glycolipid that may be formulated into cationic liposomes with or without poly(ethylene glycol) decoration. Lipoplexes formed with plasmid DNA are nuclease resistant and are targeted to the human hepatoblastoma cell line HepG2 by selective asialoglycoprotein receptor mediation. Transfection levels achieved by lipoplexes containing the targeting ligand cholesteryl-3β-N-(4-aminophenyl-β-D-galactopyranosyl) carbamate were sixfold greater than those obtained with similar but untargeted lipoplexes. |
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| Keywords: | Hepatocyte targeted PEGylated DNA lipoplexes gene transfer |
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