Transient Expression of Drosophila melanogaster Deoxynucleoside Kinase Gene Enhances Cytotoxicity of Nucleoside Analogs |
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| Authors: | Hiroyuki Kamiya Hiroshi Ochiai Hideyoshi Harashima Mana Ito Akira Matsuda |
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| Institution: | 1. Graduate School of Pharmaceutical Sciences, Hokkaido University , Kita-ku , Sapporo , Japan;2. CREST, Japan Science and Technology , Japan;3. Graduate School of Pharmaceutical Sciences, Hokkaido University , Kita-ku , Sapporo , Japan |
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| Abstract: | The Drosophila melanogaster deoxynucleoside kinase gene was introduced into HeLa cells with cationic lipids to allow its transient expression, and cytotoxic effects of several nucleoside analogs in the transfected cells were examined. Of the analogs tested, cytotoxicities of 1-β-D-arabinofuranosylcytosine (araC), 5-fluorodeoxyuridine (FUdR), and 1-(2-deoxy-2-methylene-β-D-erythro-pentofuranosyl)cytosine (DMDC) were increased by the deoxynucleoside kinase gene. These results suggest that the combination of the transient expression of the Drosophila deoxynucleoside kinase gene and these nucleoside analogs is a candidate for the suicide gene therapy. |
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| Keywords: | Deoxynucleoside kinase Nonviral vector Nucleoside analog Suicide gene therapy |
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