Enhanced Activity of Deoxycytidine Kinase After Pulsed Low Dose Rate and Single Dose Gamma Irradiation |
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| Authors: | J. Sigmond J. Haveman N. Castro Kreder W. J. Loves C. van Bree N. A. Franken |
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| Affiliation: | 1. Department of Medical Oncology , VU University Medical Center , Amsterdam, the Netherlands;2. Department of Radiotherapy , Academic Medical Center , Amsterdam, the Netherlands |
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| Abstract: | In both pulsed low dose rate (LDR) and single high dose radiation schedules, gemcitabine pretreatment sensitizes tumor cells to radiation. These radiosensitizing effects could be the result of decreased DNA repair. In this study, the effect of irradiation on the deoxycytidine kinase (dCK) needed for DNA repair was investigated. The activity of dCK, a deoxynucleoside analogue-activating enzyme was increased upon irradiation in both schedules. No change in dCK protein expression was observed that indicates a post-translational regulation. The benefit of this increased activity induced by irradiation should be further investigated in combination with deoxynucleoside analogues activated by this enzyme. |
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| Keywords: | Radiation Deoxycytidine kinase |
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