Studying the Influence of the Pyrene Intercalator TINA on the Stability of DNA i-Motifs |
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| Authors: | Ahmed A El-Sayed Erik B Pedersen Nahid A Khaireldin |
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| Institution: | 1. Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy , University of Southern Denmark , Odense M , Denmark;2. Photochemistry Department , Chemical Industries Division, National Research Center , Dokki , Giza , Egypt |
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| Abstract: | Certain cytosine-rich (C-rich) DNA sequences can fold into secondary structures as four-stranded i-motifs with hemiprotonated base pairs. Here we synthesized C-rich TINA-intercalating oligonucleotides by inserting a nonnucleotide pyrene moiety between two C-rich regions. The stability of their i-motif structures was studied by using UV melting temperature measurements and circular dichroism spectra at different pH values under noncrowding and crowding conditions (20% poly(ethylene glycol)). When TINA ((R)-3-((4-(1-pyrenylethynyl)benzyl)oxy) propane-1,2-diol) is inserted, the oligonucleotides could form an i-motif at a higher pH than observed for the corresponding wildtype oligonucleotide. |
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| Keywords: | i-Motif cytosine-rich DNA TINA-intercalating oligonucleotides crowding condition pyrene |
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