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Triplex Formation Using Oligonucleotide Clamps Carrying 8-Aminopurines |
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| Authors: | Anna Aviñó Marta G Grimau Margarita Alvira Ramon Eritja Raimundo Gargallo Modesto Orozco |
| Institution: | 1. Instituto de Biologia Molecular de Barcelona, C.S.I.C. , Barcelona , Spain;2. Institute for Research in Biomedicine (IRB), Barcelona. Parc Científic de Barcelona , Barcelona , Spain;3. Facultat de Química , Universitat de Barcelona , Barcelona , Spain;4. Institute for Research in Biomedicine (IRB), Barcelona. Parc Científic de Barcelona , Barcelona , Spain;5. Facultat de Química , Universitat de Barcelona , Barcelona , Spain |
| Abstract: | The synthesis and properties of triplex-forming DNA clamps carrying 8-aminopurines are described. The stability of triple helices is enhanced by replacing purine bases with 8-aminopurine residues. These enhanced binding properties are used for the specific capture of polypyrimidine RNA/DNA sequences of interest. |
| Keywords: | Oligonucleotide clamps 8-aminopurines binding properties triplex |