Tailoring the Peptide-Binding Specificity Of An RNA by Combinations of Specificity-Altering Mutations |
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Authors: | Maki Sugaya Futoshi Nishimura Akira Katoh Kazuo Harada |
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Institution: | 1. Department of Materials and Life Science, Faculty of Science and Technology , Seikei University , Musashino, Tokyo, Japan;2. Department of Life Sciences , Tokyo Gakugei University , Koganei, Tokyo, Japan |
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Abstract: | In this study, the ability to tailor the peptide-binding specificity of an RNA was investigated. First, variants of the Rev-response element (RRE) RNA with different specificities toward the natural binding partner, Rev, and two RRE-binding aptamers, the RSG-1.2 and the Kl peptides, were identified. Next, hybrid RRE mutants with combinations of two sets of specificity-altering substitutions were tested for peptide-binding specificity. It was shown that in most cases the results of the combination of individual mutations were of an additive nature, therefore providing a way to manipulate the peptide-binding specificity of an RNA in a predictable manner. |
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Keywords: | HIV RRE RNA arginine-rich peptide bacterial reporter system peptide-binding specificity rational design |
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