Suppression Of Hepatitis C Virus Core Protein By Short Hairpin Rna Expression Vectors In The Core Protein Expression Huh-7 Cells |
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Authors: | Hitoshi Suzuki Hiroyasu Kaneko Nobushige Tamai Kunitada Shimotohno Naoko Miyano-Kurosaki Hiroshi Takaku |
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Affiliation: | 1. Department of Life and Environmental Sciences , Chiba Institute of Technology , Chiba, Japan;2. Department of Viral Oncology , Institute for Virus Research, Kyoto University , Kyoto, Japan;3. Department of Life and Environmental Sciences , Chiba Institute of Technology , Chiba, Japan;4. High Technology Research Center , Chiba Institute of Technology , Chiba, Japan |
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Abstract: | Short hairpin RNAs (shRNAs) efficiently inhibit gene expression by RNA interference. Here, we report the efficient inhibition by DNA-based vector-derived shRNAs of core protein expression in Huh-7 cells. The shRNAs were designed to target the core region of the hepatitis C virus (HCV) genome. The core region is the most conserved region in the HCV genome, making it an ideal target for shRNAs. We identified an effective site on the core region for suppression of the HCV core protein. The HCV core protein in core protein-expressing Huh-7 cells was downregulated by core protein-shRNA expression vectors (core-shRNA-452, 479, and 503). Our results support the feasibility of using shRNA-based gene therapy to inhibit HCV core protein production. |
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Keywords: | RNAi Short hairpin RNAs (shRNAs) hepatitis C virus (HCV) core protein Huh-7 cells GFP DsRed |
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