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A Simple Fluorescent Biosensor for Theophylline Based on its RNA Aptamer |
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| Authors: | C. J. Rankin E. N. Fuller K. H. Hamor S. A. Gabarra T. P. Shields |
| Affiliation: | 1. Department of Chemistry and Biochemistry , University of North Carolina-Greensboro , Greensboro, North Carolina, USA;2. Department of Chemistry and Biochemistry , University of San Diego , San Diego, California, USA;3. Department of Chemistry and Biochemistry , University of North Carolina-Greensboro , Greensboro, North Carolina, USA;4. Department of Chemistry and Biochemistry , University of San Diego , San Diego, California, USA |
| Abstract: | Theophylline is a potent bronchodilator with a narrow therapeutic index. A simple fluorescent biosensor that detects clinically relevant theophylline concentrations has been developed using the well-characterized theophylline binding RNA aptamer. Hybridization of the RNA aptamer to a fluorescently labeled DNA strand (FL-DNA) yields a fluorescent RNA:DNA hybrid that is sensitive to theophylline. The biosensor retains the remarkable selectivity of the RNA aptamer for theophylline over caffeine and is sensitive to 0–2 μM theophylline, well below the clinically relevant concentration (5–20 mg/L or ～10–50 μM). Adding a dabcyl quenching dye to the 3′-terminus of the fluorescently labeled DNA strand yielded a dual-labeled DNA strand (FL-DNA-Q) and increased the dynamic range of this simple biosensor from 1.5-fold to 4-fold. |
| Keywords: | RNA aptamer Fluorescent biosensor Theophylline |