Production of protective antibodies against Porphyromonas gingivalis strains by immunization with recombinant gingipain domains |
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Authors: | Yasaki-Inagaki Yuriko Inagaki Satoru Yamada Satoru Okuda Katsuji Ishihara Kazuyuki |
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Affiliation: | Department of Periodontics, Tokyo Dental College, Chiba, Japan. |
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Abstract: | We studied the effect of antibodies against Porphyromonas gingivalis gingipain domains, preparing them against three recombinant fragments of RgpA (catalytic domain, r-Rgp CAT; hemagglutinin domains, r-Rgp 44 and r-Rgps 15-27) and one fragment of Kgp (catalytic domain, r-Kgp CAT). Enhancement of opsonization and killing by human polymorphonuclear leukocytes were measured in the noninvasive FDC 381 and invasive W50 strains of P. gingivalis. Anti-r-Rgp 44 was the most effective in both strains of P. gingivalis. The present findings lead us to recommend RgpA 44 as a candidate immunogen for vaccines against P. gingivalis. |
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Keywords: | Porphyromonas gingivalis gingipain vaccine protease opsonization polymorphonuclear cell |
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