Progress-curve equations for reversible enzyme-catalysed reactions inhibited by tight-binding inhibitors. |
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Authors: | S E Szedlacsek V Ostafe R G Duggleby M Serban M O Vlad |
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Affiliation: | Department of Biochemistry, Institute of Biological Sciences, Bucharest, Romania. |
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Abstract: | The rate equation for a tight-binding inhibitor of an enzyme-catalysed first-order reversible reaction was used to derive two integrated equations. One of them covers the situations in which competitive, uncompetitive or non-competitive inhibition occurs and the other refers to the special non-competitive case where the two inhibition constants are equal. For these equations, graphical and non-linear regression methods are proposed for distinguishing between types of inhibition and for calculating inhibition constants from progress-curve data. The application of the non-linear regression to the analysis of stimulated progress curves in the presence of a tight-binding inhibitor is also presented. The results obtained are valid for any type of 'dead-end'-complex-forming inhibitor and can be used to characterize an unknown inhibitor on the basis of progress curves. |
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