Elimination of residual RNase activity from purified preparations of RNA-directed DNA polymerase. |
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Authors: | M S Collett A J Faras |
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Affiliation: | Department of Microbiology University of Minnesota Medical School Minneapolis, Minnesota 55455 USA |
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Abstract: | Preparations of RNA-directed DNA polymerase purified from RNA tumor viruses by standard methods generally contain trace amounts of single-stranded RNA endonucleolytic activity detectable only by relatively sensitive methods. This contaminating RNase activity has been found to be completely inhibited when RNA-directed DNA polymerase reactions are carried out in the presence of low concentrations of bentonite. Under these conditions, only minimal inhibition of the DNA polymerase and RNase H activities of the RNA-directed DNA polymerase was observed. |
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