A nitrate-ethanol synthetic accumulation medium for isolating Pseudomonas aeruginosa from the water of surface reservoirs and sewage

A synthetic accumulation medium containing ethanol as the source of carbon and energy and potassium nitrate as the source of nitrogen is proposed. Potassium nitrate, serving as electron acceptor for P. aeruginosa, ensures its growth under anaerobic conditions, which enhances the selective properties of the medium. Comparison with Bonde citrate synthetic medium, broth with malachite green and glucose-peptone medium has shown the advantages of the proposed medium.