DNA fingerprinting—a valuable new technique for the characterisation of cell lines |
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Authors: | Glyn Stacey Bryan Bolton Alan Doyle Bryan Griffiths |
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Institution: | (1) European Collection of Animal Cell Cultures and Animal Cell Technology, Biologics Division, PHLS-CAMR, 5P4 OJG, Porton Down, Wiltshire, UK |
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Abstract: | DNA fingerprinting is an important new development for the authentication of cell lines. Multilocus methods such as those
developed by Alec Jeffreys provide information on a wide range of genetic loci throughout the human genome and thus give a
useful genetic “snap-shot” of a cell culture. Our work has shown that Jeffreys multilocus fingerprinting method can be applied
to cell lines from a wide range of animals including reptiles, birds, fish and diverse mammals. It can also differentiate
very closely related cell lines including those from the same mouse strain. Routine fingerprint analysis has enabled an unprecedented
level of confidence in the consistency of cell stocks. Our results demonstrate that this straightforward method represents
a powerful and readily interpreted system for cell authentication and exclusion of cross-contamination. |
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Keywords: | DNA fingerprinting cell lines authentication |
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