Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii |
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| Institution: | 1. Bielefeld University, Faculty of Biology, Center for Biotechnology (CeBiTec), Universitätsstrasse 27, 33615 Bielefeld, Germany;2. Biomolecular Photonics, Department of Physics, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany;1. School of Environmental Science and Engineering, Kochi University of Technology (KUT), Tosayamada, Kochi 782-8502, Japan;2. Carnegie Institution for Science, 260 Panama St. Stanford, CA 94305, USA;3. Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agricultural & Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan;4. Japan Science and Technology Agency (JST), 5 Sanbancho, Chiyoda, Tokyo 102-0075, Japan;1. Bielefeld University, Faculty of Biology, Center for Biotechnology (CeBiTec), Universitätsstrasse 27, 33615 Bielefeld, Germany;2. Institute of Biotechnological Process Engineering, Faculty of Engineering and Mathematics, University of Applied Sciences, Universitätsstrasse 27, 33615 Bielefeld, Germany;3. Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Sciences, Section I: Food Process Engineering, Kaiserstraße 12, 76131 Karlsruhe, Germany;4. Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Sciences, Section III: Bioprocess Engineering, Fritz-Haber-Weg 2, 76131 Karlsruhe, Germany;1. School of Environmental Science and Engineering, Kochi University of Technology (KUT), Tosayamada, Kochi 782-8502, Japan;2. Japan Science and Technology Agency, CREST, 5, Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan;1. Institute of Technical Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany;2. Institute of Food Chemistry, Leibniz University of Hannover, Callinstr. 5, 30167 Hannover, Germany;1. Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 08826, Republic of Korea;2. School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea;3. Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea;4. School of Mechanical and Aerospace Engineering, Seoul National University, Seoul 08826, Republic of Korea;5. Institute of Advanced Mechanics and Design, Seoul National University, Seoul 08826, Republic of Korea |
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| Abstract: | The heterologous expression of terpene synthases in microbial hosts has opened numerous possibilities for bioproduction of desirable metabolites. Photosynthetic microbial hosts present a sustainable alternative to traditional fermentative systems, using freely available (sun)light and carbon dioxide as inputs for bio-production. Here, we report the expression of a patchoulol synthase from Pogostemon cablin Benth in the model green microalga Chlamydomonas reinhardtii. The sesquiterpenoid patchoulol was produced from the alga and was used as a marker of sesquiterpenoid production capacity. A novel strategy for gene loading was employed and patchoulol was produced up to 922±242 µg g?1 CDW in six days. We additionally investigated the effect of carbon source on sesquiterpenoid productivity from C. reinhardtii in scale-up batch cultivations. It was determined that up to 1.03 mg L?1 sesquiterpenoid products could be produced in completely photoautotrophic conditions and that the alga exhibited altered sesquiterpenoid production metabolism related to carbon source. |
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| Keywords: | Microalgae Terpenoids Sesquiterpenoids Patchoulol |
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