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Natural variation of MS2 confers male fertility and drives hybrid breeding in soybean |
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| Authors: | Xiaolong Fang Xiangchi Feng Xiaoyuan Sun Xiangdong Yang Qing Li Xulei Yang Jie Xu Minghui Zhou Chunjing Lin Yi Sui Limei Zhao Baohui Liu Fanjiang Kong Chunbao Zhang Meina Li |
| Institution: | 1. Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, China
These authors contributed equally.;2. Key Laboratory of Hybrid Soybean Breeding of the Ministry of Agriculture and Rural Affairs, Jilin Academy of Agricultural Sciences, Changchun, China These authors contributed equally.;3. State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China;4. Key Laboratory of Hybrid Soybean Breeding of the Ministry of Agriculture and Rural Affairs, Jilin Academy of Agricultural Sciences, Changchun, China;5. Core Facility and Technical Service Center for SLSB, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China;6. Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design, Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, China |
| Abstract: | A complete and genetically stable male sterile line with high outcrossing rate is a prerequisite for the development of commercial hybrid soybean. It was reported in the last century that the soybean male sterile ms2 mutant has the highest record with seed set. Here we report the cloning and characterization of the MS2 gene in soybean, which encodes a protein that is specifically expressed in the anther. MS2 functions in the tapetum and microspore by directly regulating genes involved in the biosynthesis of secondary metabolites and the lipid metabolism, which is essential for the formation of microspore cell wall. Through comparison of the field performance with the widely used male sterile mutants in the same genetic background, we demonstrated that the ms2 mutant conducts the best in outcrossing rate and makes it an ideal tool in building a cost-effective hybrid system for soybean. |
| Keywords: | heterosis male sterility soybean pollen wall |