Metabolic engineering of Corynebacterium glutamicum for the de novo production of ethylene glycol from glucose |
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| Institution: | 1. Institute of Applied Chemistry, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;2. Tsinghua Innovation Center in Dongguan, Dongguan 523808, China;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;2. Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai 200237, China;1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China;2. National Engineering Laboratory for Cereal Fermentation Technology (NELCF), Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China;3. School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China;2. Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi, China;3. Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, China;1. Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea;2. BioInformatics Research Center, KAIST, Daejeon 34141, Republic of Korea;3. BioProcess Engineering Research Center, KAIST, Daejeon 34141, Republic of Korea;1. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China;3. Engineering Systems Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;1. Frontier Research Laboratories, Institute for Innovation, Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan;2. Department of Microbial Resources, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, Japan;3. Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, Japan;4. Microbial Genomics Laboratory, New Industry Creation Hatchery Center, Tohoku University, Sendai, Miyagi 980-8579, Japan |
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| Abstract: | Development of sustainable biological process for the production of bulk chemicals from renewable feedstock is an important goal of white biotechnology. Ethylene glycol (EG) is a large-volume commodity chemical with an annual production of over 20 million tons, and it is currently produced exclusively by petrochemical route. Herein, we report a novel biosynthetic route to produce EG from glucose by the extension of serine synthesis pathway of Corynebacterium glutamicum. The EG synthesis is achieved by the reduction of glycoaldehyde derived from serine. The transformation of serine to glycoaldehyde is catalyzed either by the sequential enzymatic deamination and decarboxylation or by the enzymatic decarboxylation and oxidation. We screened the corresponding enzymes and optimized the production strain by combinatorial optimization and metabolic engineering. The best engineered C. glutamicum strain is able to accumulate 3.5 g/L of EG with the yield of 0.25 mol/mol glucose in batch cultivation. This study lays the basis for developing an efficient biological process for EG production. |
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| Keywords: | Ethylene glycol Serine Metabolic engineering Pathway optimization |
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