Biological conversion of gaseous alkenes to liquid chemicals |
| |
| Affiliation: | 1. School of Public Health, University of California, Berkeley, CA, United States;2. Electric Power Research Institute, Palo Alto, CA, United States;1. Post-doctoral Fellow, Petroleum Systems Engineering, University of Regina, Regina, SK, Canada;2. Professor, Petroleum Systems Engineering, University of Regina, Regina, SK, Canada;1. Department of Civil and Environmental Engineering, The Pennsylvania State University, University Park, PA 16802, USA;2. Biological and Environmental Sciences and Engineering Division, Water Desalination and Reuse Research Center, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia;3. Department of Civil and Environmental Engineering, Southern Methodist University, Dallas, TX 75205, USA;1. School of Natural and Environmental Sciences, Bedson Building, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK;2. NewChem Technologies, The Biosphere, Draymans Way, Newcastle Helix, Newcastle upon Tyne, NE4 5BX, UK;3. Shell International Chemicals BV, Shell Research and Technology Centre Amsterdam, Toxicology Department, P.O. Box 38000, 1030BN, Amsterdam, the Netherlands;1. School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, China;2. National Engineering Laboratory of Coal-Fired Pollutants Emission Reduction, Shandong University, Jinan, Shandong, China |
| |
| Abstract: | Industrial gas-to-liquid (GTL) technologies are well developed. They generally employ syngas, require complex infrastructure, and need high capital investment to be economically viable. Alternatively, biological conversion has the potential to be more efficient, and easily deployed to remote areas on relatively small scales for the utilization of otherwise stranded resources. The present study demonstrates a novel biological GTL process in which engineered Escherichia coli converts C2–C4 gaseous alkenes into liquid diols. Diols are versatile industrially important chemicals, used routinely as antifreeze agents, polymer precursors amongst many other applications. Heterologous co-expression of a monooxygenase and an epoxide hydrolase in E. coli allows whole cell conversion of C2–C4 alkenes for the formation of ethylene glycol, 1,2-propanediol, 1,2-butanediol, and 2,3-butanediol at ambient temperature and pressure in one pot. Increasing intracellular NADH supply via addition of formate and a formate dehydrogenase increases ethylene glycol production titers, resulting in an improved productivity of 9 mg/L/h and a final titer of 250 mg/L. This represents a novel biological method for GTL conversion of alkenes to industrially valuable diols. |
| |
| Keywords: | Biological gas to liquid conversion Metabolic engineering Alkenes Diols |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
