The effect of individual genetic heterozygosity on general homeostasis,heterosis and resilience in Siberian larch (Larix sibirica Ledeb.) using dendrochronology and microsatellite loci genotyping |
| |
| Affiliation: | 1. Khakasia Technical Institute, Siberian Federal University, 27 Shchetinkina St., Abakan 655017, Russia;2. Siberian Federal University, Pr. Svobodniy 79, Krasnoyarsk 660041, Russia;3. V.N. Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk 660036, Russia;4. Georg-August-University of Göttingen, Büsgenweg 2, D-37077 Göttingen, Germany;5. N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina St., Moscow 119333, Russia;1. Department of Chemistry, Siberian Federal University, 79 Svobodny Aven., Krasnoyarsk 660041, Russia;2. Laboratory of Crystal Physics, Kirensky Institute of Physics, SB RAS, bld. 38, Akademgorodok 50, Krasnoyarsk 660036, Russia;3. Department of Physics, Far Eastern State Transport University, 47 Seryshev Str., Khabarovsk 680021, Russia;4. Laboratory of Optical Materials and Structures, Institute of Semiconductor Physics, 13 Lavrentiev Aven., Novosibirsk 630090, Russia;5. Functional Electronics Laboratory, Tomsk State University, 36 Lenin Aven., Tomsk 634050, Russia;6. Laboratory of Semiconductor and Dielectric Materials, Novosibirsk State University, 2 Pirogov Str., Novosibirsk 630090, Russia;1. Norwegian University of Science and Technology, Department of Energy and Process Engineering, Trondheim, Norway;2. SINTEF Materials and Chemistry, Flow Technology Department, Trondheim, Norway;3. Chemical Process Intensification, Eindhoven University of Technology, Department of Chemical Engineering and Chemistry, Eindhoven, The Netherlands;1. Büsgen-Institut – Forstgenetik und Forstpflanzenzüchtung, Universität Göttingen, Büsgenweg 2, 37077 Göttingen, Germany;2. Institut für ökologische und Populationsgenetik, Am Pfingstanger 58, 37075 Göttingen, Germany;1. Department of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, 60131 Ancona, Italy;2. Fondazione Fojanini, 23100 Sondrio, Italy;3. CREA—Council for Agricultural Research and Economics—Fruit research centre, 00134 Rome, Italy;1. Siberian Federal University, Svobodny pr., 79/1, Krasnoyarsk 660041, Russia;2. Kirensky Institute of Physics Federal Research Center KSC Siberian Branch Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk 660036 Russia;1. Siberian Federal University, 79 Svobodny pr., Krasnoyarsk 660041, Russia;2. L.V. Kirensky Institute of Physics, 50 Akademgorodok, Krasnoyarsk 660036, Russia;3. Kyungpook National University, 80 Daehakro, Bukgu, Daegu 41566, Republic of Korea;4. National University of Science and Technology MISiS, 4 Leninskiy prospekt, Moscow 119049, Russia |
| |
| Abstract: | The genetic mechanisms underlying the relationship of individual heterozygosity (IndHet) with heterosis and homeostasis are not fully understood. Such an understanding, however, would have enormous value as it could be used to identify trees better adapted to environmental stress. Dendrochronology data, in particular the individual average radial increment growth of wood measured as the average tree ring width (AvTRW) and the variance of tree ring width (VarTRW) were used as proxies for heterosis (growth rate measured as AvTRW) and homeostasis (stability of the radial growth of individual trees measured as VarTRW), respectively. These traits were then used to test the hypothesis that IndHet can be used to predict heterosis and homeostasis of individual trees. Wood core and needle samples were collected from 100 trees of Siberian larch (Larix sibirica Ledeb.) across two populations located in Eastern Siberia. DNA samples were obtained from the needles of each individual tree and genotyped for eight highly polymorphic microsatellite loci. Then mean IndHet calculated based on the genotypes of eight loci for each tree was correlated with the statistical characteristics of the measured radial growth (AvTRW and VarTRW) and the individual standardized chronologies. The analysis did not reveal significant relationships between the studied parameters. In order to account for the strong dependence of the radial growth on tree age the age curves were examined. An original approach was employed to sort trees into groups based on the distance between these age curves. No relationship was found between these groups and the groups formed based on heterozygosity. However, further work with more genetic markers and increased sample sizes is needed to test this novel approach for estimating heterosis and homeostasis. |
| |
| Keywords: | Dendrochronology Tree ring width Radial growth Individual heterozygosity Microsatellite markers Heterosis Homeostasis Climate change Environmental stress |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
