| 蛇毒神经生长因子对大鼠脊髓损伤后Caspase-3表达影响 |
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| 引用本文： | 葛洪亮,贺茂林,李世德.蛇毒神经生长因子对大鼠脊髓损伤后Caspase-3表达影响[J].蛇志,2011,23(2):111-113. |
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| 作者姓名： | 葛洪亮 贺茂林 李世德 |
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| 作者单位： | 广西医科大学第一附属医院脊柱骨病科,广西南宁,530021 |
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| 摘 要： | 目的 探讨大鼠脊髓损伤后应用蛇毒神经生长因子对Caspase-3表达的影响.方法 将55只成年SD雄性大鼠随机分为蛇毒神经生长因子组（A组）、生理盐水组（B组）、假手术组（C组）,按改良Allen打击法建立大鼠脊髓不完全损伤模型,通过动物神经运动功能BBB评分评价神经损伤程度及神经功能恢复情况;脊髓损伤后不同时间点（6 h、1 d、3 d、7 d、14 d）取材,HE染色观察损伤脊髓组织病理变化,免疫组化染色检测Caspase-3阳性细胞的表达,来比较分析两组的差异性.结果 HE染色镜检发现脊髓组织病理学改变A组明显轻于B组;BBB评分A组相对B明显提高,差异有显著性意义（P〈0.05）.A组和B组均发现凋亡细胞及Caspase-3表达,神经细胞凋亡指数及Caspase-3表达均为B组〉A组（P〈0.05）.结论蛇毒神经生长因子能抑制大鼠脊髓损伤后Caspase-3表达及细胞凋亡,能改善脊髓损伤后的功能表现.
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| 关 键 词： | 蛇毒神经生长因子 脊髓损伤 Caspase-3 BBB功能评分 |
Effect of Snake Nerve Growth Factor on Caspase-3 expression after spinal cord injury in rats |
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| GE Hong-liang,HE Mao-lin,LI Shi-de.Effect of Snake Nerve Growth Factor on Caspase-3 expression after spinal cord injury in rats[J].Journal of Snake,2011,23(2):111-113. |
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| Authors: | GE Hong-liang HE Mao-lin LI Shi-de |
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| Institution: | GE Hong-liang,HE Mao-lin,LI Shi-de(Department of Spine and Osteopathy ward,the First Affilicated Hospital of Guangxi Medical University,Nanning,Guangxi,530021,China) |
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| Abstract: | Objective To investigate the effect of Snake Nerve Growth Factor(NGF)on Caspase-3 expression after spinal cord injury(SCI) in rats.Methods Fifty-five adult SD rats were randomly divided into Snake Nerve Growth Factor(group A),normal saline(group B),sham group(group C),the SCI model was induced according to improved allen's method,the neurofunctional recovery was observed according to BBB score,after operation,the rats was sacrificed in different time(6 h,1 d,3 d,7 d,14 d),and the injuried spinal tissue spec... |
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| Keywords: | Caspase-3 |
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