| HPLC-ELSD测定抗宫炎片中盐酸水苏碱的含量 |
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| 引用本文： | 刘桂贞.HPLC-ELSD测定抗宫炎片中盐酸水苏碱的含量[J].蛇志,2011,23(2):137-140. |
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| 作者姓名： | 刘桂贞 |
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| 作者单位： | 广西医科大学第八附属医院急诊科,广西贵港,537100 |
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| 摘 要： | 目的 建立HPLC-ELSD法测定抗宫炎片中盐酸水苏碱的含量.方法采用Lichrospher 5-NH2色谱柱,流动相为乙腈-水（86∶14）,流速1.0 ml/min,柱温30℃;检测器参数：雾化室温度60℃,漂移管温度80℃,载气体积流量1.2 ml/min.结果盐酸水苏碱在2.654~13.270 μg范围内线性关系良好,r=0.9994,平均回收率为97.7%,RSD=1.9%（n=6）.结论 该法简便可行,结果准确可靠,可用于抗宫炎片中盐酸水苏碱的含量测定.
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| 关 键 词： | 抗宫炎片 盐酸水苏碱 HPLC-ELSD |
Determination of stachydrine hydrochloride in Risiting Pelvic Inflammatory Tablets by HPLC-ELSD |
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| LIU Gui-zheng.Determination of stachydrine hydrochloride in Risiting Pelvic Inflammatory Tablets by HPLC-ELSD[J].Journal of Snake,2011,23(2):137-140. |
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| Authors: | LIU Gui-zheng |
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| Institution: | LIU Gui-zheng(Department of Emergency,The Eighth Affiliated Hospital of Guangxi Medical University,Guigang,Guangxi,537100,China) |
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| Abstract: | Objective To establish a HPLC-ELSD method for the determination of stachydrine hydrochloride of Risiting Pelvic Infammatory Tablets. Methods Column. Lichrospher 5-N H2 （250 mm × 4.60 ram） ; mobile phase ： acetonitrile-water（86 ： 14）, flow rate was 1.0 ml/min,the column temperature was 30℃ ;detecter parameter： the nebulizar temperature was 60℃ ,and the evaporation temperature was 80℃ ;flow rate was 1.2 SLM. Results Under the chromatographic conditions used, the calibration curve of stachydrine hydrochloride was linear in the range of 2. 654-13. 270 μg,the average recoveries was 97.7% ,RSD：1. 77%. The average recovery was 97.7 %, RSD： 1.9% （n = 6）. Conclusion The method is accurate, effective and feasible. It can be used for the determination of the contents of stachydrine hydrochloride contained in Risiting Pelvic Inflammatory Tablets. |
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| Keywords: | HPLC-ELSD |
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