Excellent laundry detergent compatibility and high dehairing ability of the Bacillus pumilus CBS alkaline proteinase (SAPB) |
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Authors: | Bassem Jaouadi Semia Ellouz-Chaabouni Mamdouh Ben Ali Ezzedine Ben Messaoud Belgacem Naili Abdelhafidh Dhouib Samir Bejar |
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Institution: | 1. Laboratoire d’Enzymes et de Métabolites des Procaryotes (LEMP), Centre de Biotechnologie de Sfax (CBS), B.P 1177, Route Sidi Mansour Km 6, 3018, Sfax, Tunisia 2. Unité Enzymes et Bioconversion (UEB), Ecole Nationale d’Ingénieurs de Sfax (ENIS), B.P 1173, Route Soukra Km 3, 3038, Sfax, Tunisia 3. Laboratoire des Bioprocédés (LBP), Centre de Biotechnologie de Sfax (CBS), B.P 1177, Route Sidi Mansour Km 6, 3018, Sfax, Tunisia
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Abstract: | The newly Tunisian soil-isolated bacterium, producing the alkaline proteinase termed SAPB that was already purified and characterized
1], was assigned as Bacillus pumilus CBS strain on the basis of biochemical properties and 16S rRNA gene sequencing. The maximum protease activity recorded after
24 h of incubation in an optimized medium at 37°C was 6,500 U/mL in shaking flask culture and 25,000 U/mL in fermentor. SAPB
showed excellent stability and compatibility in laundry detergent retaining more than 98% of its initial activity after pre-incubation
for 1 h at 40°C with Det, followed by OMO (97%), Dinol (94%), and Dixan (93%). Examination of various stained cloth pieces
exhibited a remarkable efficiency in the removal of blood and chocolate stains. More interestingly, SAPB demonstrated powerful
dehairing capabilities of hair removal from skin with minimal damage on the collagen and a nearly complete feather-degradation.
Likewise, Bacillus pumilus CBS effectively degraded feather-meal (98.5%), chicken feather (92%), goat hair (80%), and bovine hair (68%) whereas sheep
wool under went less degradation. Keratin-degradation resulted in sulfhdryl group formation (0.95∼3.91 μM). |
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