Immunological detection of penicillin-binding protein 2' in clinical isolates of methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis |
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| Authors: | Denise M. O''Hara Charles R. Harrington Peter E. Reynolds |
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| Affiliation: | Department of Biochemistry, University of Cambridge, U.K. |
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| Abstract: | The additional penicillin-binding protein (PBP 2') that is important in determining intrinsic resistance in methicillin-resistant strains of Staphylococcus aureus (MRSA) has been detected immunologically in strains from a variety of world-wide locations. This additional protein has also been definitively identified both immunologically and as a PBP in methicillin-resistant strains of S. epidermidis (MRSE). The assay described is rapid, specific and sensitive and has been used to detect PBP 2' in S. haemolyticus but not in beta-lactam resistant Streptococci. |
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| Keywords: | Staphylococcus aureus (MRSA) S. epidermidis (MRSE) Immunoassay Penicillin-binding protein Immunoblotting Monoclonal antibody |
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