Tissue clearing‐based method for unobstructed three‐dimensional imaging of mouse penis with subcellular resolution |
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Authors: | Pawe Matryba Artur Wolny Monika Pawowska Anna Sosnowska Zuzanna Rydzyska Marcin Jasiski Marzena Stefaniuk Jakub Gob |
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Institution: | Pawe? Matryba,Artur Wolny,Monika Paw?owska,Anna Sosnowska,Zuzanna Rydzyńska,Marcin Jasiński,Marzena Stefaniuk,Jakub Go??b |
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Abstract: | Although mice are widely used to elucidate factors contributing to penile disorders and develop treatment options, quantification of tissue changes upon intervention is either limited to minuscule tissue volume (histology) or acquired with limited spatial resolution (MRI/CT). Thus, imaging method suitable for expeditious acquisition of the entire mouse penis with subcellular resolution is described that relies on both aqueous‐ (clear, unobstructed brain imaging cocktails and computational analysis) and solvent‐based (fluorescence‐preserving capability imaging of solvent‐cleared organs) tissue optical clearing (TOC). The combined TOC approach allows to image mouse penis innervation and vasculature with unprecedented detail and, for the first time, reveals the three‐dimensional structure of murine penis fibrocartilage. |
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Keywords: | light sheet penile innervation penile vasculature penis imaging tissue clearing |
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