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Assessment of the potential bactericide effect of self‐cleaning floors: a proposed protocol
Authors:Erzs  bet Szab  ‐B  rdos,Petra Kulcs  r,N  ra Kov  ts,Zs  fia B  k  ssy,Bettina Eck‐Varanka,Ott   Horv  th
Affiliation:Erzsébet Szabó‐Bárdos,Petra Kulcsár,Nóra Kováts,Zsófia Békéssy,Bettina Eck‐Varanka,Ottó Horváth
Abstract:The antibacterial properties of self‐cleaning coatings are based on bactericide nanoparticles (NPs). Ecotoxicity of these NPs have been assessed mostly in suspension, using standard bioassays. Here a protocol is proposed to test actual coating samples, using the Vibrio fischeri bioluminescence inhibition bioassay. The protocol was designed to test bactericide properties of specially coated PVC floors being used in hospital environments under quasinatural conditions, such as prolonged exposure or room temperature. To take into consideration that the light output of the bacteria under prolonged exposure naturally changes, a correction factor is proposed.
Keywords:bactericide effect  nanoparticle ecotoxicity  self‐cleaning floor  Vibrio fischeri bioassay
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