| Abstract: | A new colorimetric and fluorescent probe MNTPZ based on 1H‐imidazo4,5‐b]phenazine derivative has been designed and synthesized for successive detection of Ag+ and I?. The probe MNTPZ shows selective colorimetric response by a change in color from yellow to orange and “turn‐off” fluorometric response upon binding with Ag+ in DMSO: Water (pH = 7, 1:1, v/v) over other cations. The binding mode of probe MNTPZ to Ag+ was studied by Job's plot, 1H NMR studies, FT‐IR spectroscopy and DFT calculations. Moreover, the situ generated probe MNTPZ + Ag+ complex acted as an efficient fluorometric “turn‐on” probe for I? via Ag+ displacement approach. The detection limit of probe MNTPZ for Ag+ and the resultant complex probe MNTPZ + Ag+ for I? were determined to be 1.36 μmol/L and 1.03 μmol/L respectively. Notably, the developed probe was successfully used for quantitative determination of I? in real samples with satisfactory results. |
