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Genome sequence of the acid-tolerant Burkholderia sp. strain WSM2232 from Karijini National Park,Australia
Authors:Robert Walker  Elizabeth Watkin  Rui Tian  Lambert Br?u  Graham O’Hara  Lynne Goodwin  James Han  Tatiparthi Reddy  Marcel Huntemann  Amrita Pati  Tanja Woyke  Konstantinos Mavromatis  Victor Markowitz  Natalia Ivanova  Nikos Kyrpides  Wayne Reeve
Abstract:Burkholderia sp. strain WSM2232 is an aerobic, motile, Gram-negative, non-spore-forming acid-tolerant rod that was trapped in 2001 from acidic soil collected from Karijini National Park (Australia) using Gastrolobium capitatum as a host. WSM2232 was effective in nitrogen fixation with G. capitatum but subsequently lost symbiotic competence during long-term storage. Here we describe the features of Burkholderia sp. strain WSM2232, together with genome sequence information and its annotation. The 7,208,311 bp standard-draft genome is arranged into 72 scaffolds of 72 contigs containing 6,322 protein-coding genes and 61 RNA-only encoding genes. The loss of symbiotic capability can now be attributed to the loss of nodulation and nitrogen fixation genes from the genome. This rhizobial genome is one of 100 sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
Keywords:root-nodule bacteria  nitrogen fixation  rhizobia  Betaproteobacteria
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