β-III Tubulin Fragments Inhibit α-Synuclein Accumulation in Models of Multiple System Atrophy |
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Authors: | Yasuyo Suzuki Chenghua Jin Tamaki Iwase Ikuru Yazawa |
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Institution: | From the ‡Laboratory of Research Resources, Research Institute, National Center for Geriatrics and Gerontology, 35 Gengo, Morioka, Obu-shi, Aichi 474-8511, Japan and ;the §Department of Neurology, Nagoya City Koseiin Medical Welfare Center, Aichi 465-8610, Japan |
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Abstract: | Multiple system atrophy (MSA) is a neurodegenerative disease caused by α-synuclein aggregation in oligodendrocytes and neurons. Using a transgenic mouse model overexpressing human α-synuclein in oligodendrocytes, we previously demonstrated that oligodendrocytic α-synuclein inclusions induce neuronal α-synuclein accumulation and progressive neuronal degeneration. α-Synuclein binds to β-III tubulin, leading to the neuronal accumulation of insoluble α-synuclein in an MSA mouse model. The present study demonstrates that α-synuclein co-localizes with β-III tubulin in the brain tissue from patients with MSA and MSA model transgenic mice as well as neurons cultured from these mice. Accumulation of insoluble α-synuclein in MSA mouse neurons was blocked by the peptide fragment β-III tubulin (residues 235–282). We have determined the α-synuclein-binding domain of β-III tubulin and demonstrated that a short fragment containing this domain can suppress α-synuclein accumulation in the primary cultured cells. Administration of a short α-synuclein-binding fragment of β-III tubulin may be a novel therapeutic strategy for MSA. |
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Keywords: | alpha-Synuclein Neurodegeneration Protein Aggregation Protein-Protein Interaction Tubulin Multiple System Atrophy |
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