| 离子交换法提取茶氨酸的研究 |
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| 引用本文： | 陈瑛 钟俊辉. 离子交换法提取茶氨酸的研究[J]. 氨基酸和生物资源, 1998, 20(1): 31-34 |
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| 作者姓名： | 陈瑛 钟俊辉 |
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| 作者单位： | [1]绍兴文理学院生化系 [2]无锡轻工大学生物工程学院 |
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| 摘 要： | 本研究应用国产７３２阳离子交换树脂，从茶愈伤组织浸提液中提取茶氨酸。通过静态、动态试验，对洗脱液的ｐＨ、离子强度、样液浓度和流速等因素进行了考察，比较在不同条件下，树脂对茶氨酸的吸附情况。结果表明用ｐＨ９．１８，２／１５ｍｏｌ／ＬＮａ２ＨＰＯ４作洗脱液，控制流速在０．７ＢＶ／ｈ，可分离到茶氨酸，得率７０％。
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| 关 键 词： | 茶氨酸 732阳离子交换树脂 提取 |
Purification of Theamine with Cation Resin Chromatography |
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| Chen ying Zhong Junhui Tao Wenyi. Purification of Theamine with Cation Resin Chromatography[J]. Amino Acids & Biotic Resources, 1998, 20(1): 31-34 |
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| Authors: | Chen ying Zhong Junhui Tao Wenyi |
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| Affiliation: | Chen ying * Zhong Junhui Tao Wenyi ( *Shaoxing College of Art and Science Shaoxing 312000) (Wuxi university of Light Industry Wuxi 214036) |
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| Abstract: | Theanine were purified from the extract of tea callus with 732cation resin colum chromatognaphy. The optimal operation conditions were pH9.18,2/15mol/L Na_2HPO_4-KH_2PO_4 buffur as eluant and the elution rate 0.7BV/h by the static and dynamic state experiments.The yield of the purification was 70%. |
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| Keywords: | theanine 732cation resin extraction |
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