| Abstract: | The process of enzymatic hydrolysis of the mycelial waste from the manufacture of tetracycline with using Streptomyces aureofaciens was studied. For the enzymatic hydrolysis, neutral and alkaline proteinases were used. It was shown that alkaline proteinase (protosubtilin G10X) provided the most efficient hydrolysis. Optimal conditions for the hydrolysis were determined: a temperature of 42 degrees C, hydrolysis time of 4 to 6 hours and enzyme concentrations of 1.25 to 2.20 mg/ml at a mycecial waste concentration of 12.5 mg/ml. The time course of changes in amino acid and amine nitrogen levels during enzymatic hydrolysis was investigated. It was demonstrated that the hydrolysis efficiency depended on the mode of enzyme addition. The highest efficiency was observed with fractional feeding of the enzyme. |
